Journal: Scientific Reports

Article Title: Towards a new pseudo-quantitative approach to evaluate the ionization response of nitrogen compounds in complex matrices

doi: 10.1038/s41598-021-85854-7

Figure Lengend Snippet: GC × GC-NCD chromatograms obtained for the different types of gas oils considered. The chromatograms were obtained using a home-made software 2D Chrom.

Article Snippet: The chromatograms were obtained using a home-made software 2D Chrom.

Techniques: Software

Journal: Molecular Medicine Reports

Article Title: Expression and clinical significance of circular RNA hsa_circ_0079787 in the peripheral blood of patients with axial spondyloarthritis

doi: 10.3892/mmr.2020.11520

Figure Lengend Snippet: Snippet of the detailed annotation for circRNA/miRNA interactions. Interactions of hsa_circ_0079787/hsa-miR-580-3p, hsa_circ_0079787/hsa-miR-125b-5p, hsa_circ_0079787/hsa-miR-5191, hsa_circ_0079787/hsa-miR-6830-5p and hsa_circ_0079787/hsa-miR-4694-3p are displayed. UTR, untranslated region; miRNA/miR, microRNA; circRNA/circ, circular RNA; hsa, Homo sapiens ; M, circRNA/miRNA interaction target from Arraystar's home-made miRNA target prediction software based on miRanda; T, circRNA/miRNA interaction target from Arraystar's home-made miRNA target prediction software based on TargetScan.

Article Snippet: The circRNA/miRNA interactions were predicted using Arraystar's home-made miRNA target prediction software (version 1; KangChen Bio-tech, Inc.) ( , ) based on TargetScan (version 6) ( ) and miRanda (version 3.3a) , and the differentially expressed circRNAs within all the comparisons were annotated in detail with the circRNA/miRNA interaction information.

Techniques: Software

Journal: Molecular Therapy. Nucleic Acids

Article Title: circRASGRF2 functions as an oncogenic gene in hepatocellular carcinoma by acting as a miR-1224 sponge

doi: 10.1016/j.omtn.2020.10.035

Figure Lengend Snippet: FAK was positively regulated by circRASGRF2/miR-1224 (A) Venn diagram showing five genes that are putative miR-1224 targets computationally predicted by four algorithms (miRanda, RNAhybrid, miRWalk, and TargetScan). (B) mRNA levels of five candidate target genes were detected after silencing circRASGRF2. (C) GSEA analysis showed that the gene set of KEGG_FOCAL ADHESION_PATHWAY was significantly enriched in the si-circRASGRF2 group compared to the si-NC group. (D) Binding sequence between miR-1224 and FAK. (E) Luciferase reporter assay demonstrated that miR-1224 mimics significantly decreased the luciferase activity of FAK-WT in HEK293 T cells. (F) Inhibition of the circRASGRF2-mediated decrease of FAK mRNA and protein expression was significantly recuperated following miR-1224 inhibitors. (G) TCGA database showed that the level of FAK was significantly upregulated in HCC tissue compared with normal liver tissue. (H) The expression of FAK was downregulated in MHCC97H cells by two shRNAs. (I) MHCC97H cells transduced with FAK shRNAs had a slower growth rate. (J) The cell cycle analysis showed that more MHCC97H cells were distributed in the G 1 phase and less in the S phase after silencing FAK. (K) FAK knockdown significantly increased the percentage of apoptotic cells in MHCC97H cells. All tests were performed at least performed three times. Data are expressed as mean ± SD. ∗∗p < 0.01.

Article Snippet: The circRNA/miRNA interaction was predicted with Arraystar’s home-made software based on miRanda, PITA, and RNAhybrid.

Techniques: Binding Assay, Sequencing, Luciferase, Reporter Assay, Activity Assay, Inhibition, Expressing, Transduction, Cell Cycle Assay, Knockdown

Journal: International Journal of Molecular Medicine

Article Title: Microarray analysis of circular RNA expression patterns in polarized macrophages

doi: 10.3892/ijmm.2017.2852

Figure Lengend Snippet: A snippet of the detailed annotation for circRNA-010231/miRNA interaction. The circRNA/miRNA interaction was predicted with Arraystar's home-made miRNA target prediction software based on TargetScan and miRanda, and the most differentially expressed circRNAs within all the comparisons were annotated in detail with the circRNA/miRNA interaction information. Binding sites of conserved miRNAs with good mirSVR scores are represented.

Article Snippet: To further facilitate the implication of our research study, we used the Arraystar's home-made miRNA target prediction software based on TargetScan ( ) and miRanda ( ) to predict circRNA/microRNA interaction.

Techniques: Software, Binding Assay